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GFP‐Targeted Mitochondria Show Heterogeneity of Size, Morphology, and Dynamics in Transgenic Nicotiana tabacum L. Plants In Vivo

Yih‐Shan Lo, Lin‐June Hsiao, Wann‐Neng Jane, Yuh‐Cheng Charng, Hwa Dai and Kwen‐Sheng Chiang
International Journal of Plant Sciences
Vol. 165, No. 6 (November 2004), pp. 949-955
DOI: 10.1086/423884
Stable URL: http://www.jstor.org/stable/10.1086/423884
Page Count: 7
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GFP‐Targeted Mitochondria Show Heterogeneity of Size, Morphology, and Dynamics in Transgenic Nicotiana tabacum L. Plants In Vivo
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Abstract

This study reports on the heterogeneity and dynamics of green fluorescent protein (GFP)–tagged mitochondria in transgenic tobacco (Nicotiana tabacum) plants in vivo. We investigated the size, shape, and dynamics of mitochondria in different plant cells using confocal microscopy. The mitochondria exhibit different mobility and shapes according to their location, developmental stages, and physiological conditions. Because mitochondria in the shoot apical meristem cells vary greatly in size—from 100‐nm specks to 2‐μm spherical structures—an alternative mitochondrial propagation pathway other than fission may exist in fast‐growing eudicots. Mitochondria as small as 100 nm may undergo a progressive development in situ. Real‐time analysis demonstrated highly dynamic movement of mitochondria in vivo. We present evidence of fusion and fission of mitochondria in eudicots for the first time. We also found that some mitochondria were interconnected to each other by tubular structures and formed networks in the cell.

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