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Usefulness of Pulsed‐Field Gel Electrophoresis in Assessing Nosocomial Transmission of Pertussis

Michèle Nouvellon , MD, Jean‐François Gehanno , MD, Martine Pestel‐Caron , PhD, Christian Weber , PhD, Jean‐François Lemeland , PhD and Nicole Guiso , PhD
Infection Control and Hospital Epidemiology
Vol. 20, No. 11 (November 1999), pp. 758-760
DOI: 10.1086/501579
Stable URL: http://www.jstor.org/stable/10.1086/501579
Page Count: 3
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Usefulness of Pulsed‐Field Gel Electrophoresis in Assessing Nosocomial Transmission of Pertussis
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Abstract

ABSTRACT During a 2‐week period, three infants with a cough lasting at least 8 days with whoops, were admitted to the pediatric unit; Bordetella pertussis was isolated from nasopharyngeal aspirates collected from the three infants. Approximately 1 week later, a nurse working on the same unit developed influenza‐like symptoms followed by whooping cough; B pertussis was isolated. Isolates from the nurse and from one of the infants were shown to be indistinguishable by pulsed‐field gel electrophoresis. These data demonstrate that B pertussis transmission to healthcare workers is possible and emphasize the need to use respiratory protection devices (Droplet Precautions) for healthcare workers having close contact with infected children.

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