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Contamination of Trypan Blue With Burkholderia cepacia in a Cornea Bank
Formats Available in JSTOR: PDF
Abstract(back to top)
OBJECTIVE. To describe Burkholderia cepacia contamination of a cornea bank and the measures taken to identify and eliminate the source of infection.
METHODS. Cultures were performed to assess the extent and source of contamination, and pulsed‐field gel electrophoresis was used for molecular typing.
RESULTS. Routine surveillance cultures identified 5 contaminated corneas during a 10‐day period. Additional cultures showed that 28 of 88 samples were positive for this organism. Environmental investigation showed that an open bottle of trypan blue used to assess corneal morphology was contaminated with the epidemic strain.
CONCLUSION. Trypan blue played a major role in this contamination of corneas. This episode shows that microbial contamination can affect transplanted corneas despite ongoing culture surveillance and suggests that new methods may be needed to avoid this risk.
Bibliographic Information(back to top)
- Contamination of Trypan Blue With Burkholderia cepacia in a Cornea Bank
- Pascal C. Morel , MD, Nora Roubi , PhD, Daniel R. Talon , PhD and Xavier Bertrand , PhD
- Infection Control and Hospital Epidemiology
- Vol. 24, No. 3 (March 2003) (pp. 198-201)
Notes and References(back to top)
This item contains 1 note(s).
Notes
Drs. Morel and Roubi are from Unité de Thérapie Cellulaire et Génique, Etablissement Fran�ais du Sang, Besan�on, France. Drs. Talon and Bertrand are from Service d’Hygi�ne hospitali�re et d’Epidémiologie moléculaire, Centre Hospitalier Universitaire Jean Minjoz, Besan�on, France. Address reprint requests to Xavier Bertrand, MD, Service d’Hygi�ne hospitali�re et d’Epidémiologie moléculaire, Centre Hospitalier Universitaire Jean Minjoz, 25030 Besan�on, France.
Items Citing this Item (back to top)
2 item(s) in JSTOR cite this item
- I. Mateos , MD; R. Valencia , MD; M. J. Torres , PhD; A. Cantos , MD; M. Conde , MD; J. Aznar , MD, PhDVol. 27, No. 11 (November 2006) pp. 1249-1251Stable URL: http://www.jstor.org/stable/10.1086/508838
- Michelle Jacobson; Rick Wray , BA; Danuta Kovach , BA; Deborah Henry , BMLSc; David Speert , MD; Anne Matlow , MD, MScVol. 27, No. 4 (April 2006) pp. 362-366Stable URL: http://www.jstor.org/stable/10.1086/503343