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Journal Article

Can a Botanic Garden Cycad Collection Capture the Genetic Diversity in a Wild Population?

M. Patrick Griffith, Michael Calonje, Alan W. Meerow, Freddy Tut, Andrea T. Kramer, Abby Hird, Tracy M. Magellan and Chad E. Husby
International Journal of Plant Sciences
Vol. 176, No. 1 (January 2015), pp. 1-10
DOI: 10.1086/678466
Stable URL:
Page Count: 10
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Premise of research. Conservation of plant species often requires ex situ (off-site) cultivation of living collections. Cycads constitute the most imperiled major group of plants, and ex situ collections are an important part of conservation planning for this group, given seed recalcitrance, difficulties with tissue culture, and ongoing in situ threats. Very little is known about the genetics of ex situ conservation collections of cycads. Thus, this study seeks to illuminate how well an ex situ collection of a cycad can capture the diversity in a wild population.Methodology. A model species, Zamia decumbens, was chosen on the basis of geographic isolation and detailed census knowledge, which allowed near-total sampling of in situ plants. Overall, 375 in situ plants were compared to 205 ex situ plants via 10 microsatellite markers.Pivotal results. Genetic-distance analysis shows high fidelity of the ex situ collections to their in situ source populations as well as clustering of ex situ progeny by accession and strong identity with their respective mother plants. Structured resampling of allele capture from the in situ populations by the ex situ collections shows that allele capture increases as number of ex situ plants maintained increases, but with a diminishing rate of increase.Conclusions. These data demonstrate that botanic garden collections can better conserve the genetic diversity of in situ cycad populations if four recommendations are followed: (1) use the species biology to inform the collecting strategy; (2) manage each population separately; (3) collect and maintain multiple accessions; and (4) collect over multiple years.

Notes and References

This item contains 63 references.

Literature Cited
  • ['Baillie JEM, C Hilton-Taylor, SN Stuart, eds\u20022004\u20022004 IUCN Red List of threatened species: a global species assessment. International Union for Conservation of Nature, Gland, Switzerland.']
  • ['Berjak P, NW Pammenter\u20022014\u2002Cryostorage of germplasm of tropical recalcitrant-seeded species: approaches and problems. Int J Plant Sci 175:29–39.']
  • ['Brown AH, JD Briggs\u20021991\u2002Sampling strategies for genetic variation in ex situ collections of endangered plant species. Pages 99–119 in DA Falk, KE Holsinger, eds. Genetics and conservation of rare plants. Oxford University Press, New York.']
  • ['Calonje C, C Husby, M Calonje\u20022010\u2002Germination and early seedling growth of rare Zamia spp. in organic and inorganic substrates: advancing ex situ conservation horticulture. HortScience 45:679–683.']
  • ['Calonje M, J Kay, MP Griffith\u20022011\u2002Propagation of cycad collections from seed: applied reproductive biology for conservation. Sibbaldia 9:77–96.']
  • ['Calonje M, J Meerman, P Griffith, G Hoese\u20022009\u2002A new species of Zamia (Zamiaceae) from the Maya Mountains of Belize. J Bot Res Inst Tex 31:31–41.']
  • ['Calonje M, AW Meerow, L Knowles, D Knowles, MP Griffith, K Nakamura, J Francisco-Ortega\u20022013\u2002Cycad biodiversity in the Bahamas Archipelago and conservation genetics of the critically endangered Zamia lucayana (Zamiaceae). Oryx 47:190–198.']
  • ['Chavez VM, RE Litz\u20021999\u2002Organogenesis from megagametophyte and zygotic embryo explants of the gymnosperm Dioon edule Lindley (Zamiaceae, Cycadales). Plant Cell Tissue Organ Cult 58:219–222.']
  • ['Chavez VM, RE Litz, M Monroy, PA Moon, AM Vovides\u20021998\u2002Regeneration of Ceratozamia euryphyllidia (Cycadales, Gymnospermae) plants from embryogenic leaf cultures derived from mature-phase trees. Plant Cell Rep 17:612–616.']
  • ['Cibrián-Jaramillo A, AC Daly, E Brenner, R Desalle, TE Marler\u20022010\u2002When north and south don’t mix: genetic connectivity of a recently endangered oceanic cycad, Cycas micronesica, in Guam using EST-microsatellites. Mol Ecol 19:2364–2379.']
  • ['Cibrián-Jaramillo A, A Hird, N Oleas, H Ma, AW Meerow, J Francisco-Ortega, MP Griffith\u20022013\u2002What is the conservation value of a plant in a botanic garden? using indicators to improve management of ex situ collections. Bot Rev 79:559–577.']
  • ['Clark DA, DB Clark\u20021987\u2002Temporal and environmental patterns of reproduction in Zamia skinneri, a tropical rain forest cycad. J Ecol 75:135–149.']
  • ['Clark DB, DA Clark\u20021988\u2002Leaf production and the cost of reproduction in the Neotropical rain forest cycad, Zamia skinneri. J Ecol 76:1153–1163.']
  • ['Cohen JI, JT Williams, DL Plucknett, H Shands\u20021991\u2002Ex situ conservation of genetic resources: global development and environmental concerns. Science 253:866–872.']
  • ['Da Silva JM, JS Donaldson, G Reeves, TA Hedderson\u20022012\u2002Population genetics and conservation of critically small cycad populations: a case study of the Albany cycad, Encephalartos latifrons (Lehmann). Biol J Linn Soc 105:293–308.']
  • ['Donaldson JS, ed\u20022003\u2002Cycads: status survey and conservation action plan. International Union for Conservation of Nature, Gland, Switzerland.']
  • ['Dosmann MS\u20022006\u2002Research in the garden: averting the collections crisis. Bot Rev 72:207–234.']
  • ['Frankham R\u20021995\u2002Inbreeding and extinction: a threshold effect. Conserv Biol 9:792–799.']
  • ['Gale JS, MJ Lawrence\u20021984\u2002The decay of variability. Pages 77–85 in JHW Holden, JT Williams, eds. Crop genetic resources: conservation and evaluation. Allen & Unwin, London.']
  • ['Gilbert N\u20022010\u2002Threats to the world’s plants assessed. Nature. doi:10.1038/news.2010.499.']
  • ['González-Astorga J, F Vergara-Silva, AP Vovides, F Nicolalde-Morejón, D Cabrera-Toledo, MA Pérez-Farrera\u20022008\u2002Diversity and genetic structure of three species of Dioon Lindl. (Zamiaceae, Cycadales) from Pacific seaboard of Mexico. Biol J Linn Soc 94:765–776.']
  • ['Griffith P, C Lewis, J Francisco-Ortega\u20022011\u2002Palm conservation in a botanic garden: a case study of the Keys thatch palm. Palms 55:93–101.']
  • ['Guerrant EO Jr, P Fiedler, K Havens, M Maunder\u20022004\u2002Revised genetic sampling guidelines for conservation collections of rare and endangered plants. Pages 419–441 in EO Guerrant Jr, K Havens, M Maunder, eds. Ex situ plant conservation: supporting species survival in the wild. Island, Washington, DC.']
  • ['Guerrant EO Jr, K Havens, P Vitt\u20022014\u2002Sampling for effective ex situ plant conservation. Int J Plant Sci 175:11–20.']
  • ['Havens KA, T Kramer, EO Guerrant Jr\u20022014\u2002Getting plant conservation right (or not): the case of the United States. Int J Plant Sci 175:3–10.']
  • ['Hedrick P, S Kalinowski\u20022000\u2002Inbreeding depression in conservation biology. Annu Rev Ecol Syst 31:139–162.']
  • ['Huff DR, R Peakall, PE Smouse\u20021993\u2002RAPD variation within and among natural populations of outcrossing buffalograss Buchloe dactyloides (Nutt) Engelm. Theor Appl Genet 86:927–934.']
  • ['Husby CE, MA Calonje, L Noblick, MP Griffith\u20022007\u2002Montgomery Botanical Center collections policy. Version 2.1.']
  • ['Hyams DG\u20022014\u2002CurveExpert Professional.']
  • ['International Union for Conservation of Nature (IUCN)\u20022013\u2002IUCN Red List of threatened species. Version 2013.2. Accessed April 28, 2014.']
  • ['Kay J, A Strader, V Murphy, L Nghiem-Phu, M Calonje, MP Griffith\u20022011\u2002Palma corcho: a case study in botanic garden conservation horticulture and economics. HortTechnology 21:474–481.']
  • ['Krishnan S, TA Ranker, AP Davis, JJ Rakotomalala\u20022013\u2002The study of genetic diversity patterns of Coffea commersoniana, an endangered coffee species from Madagascar: a model for conservation of other littoral forest species. Tree Genet Genomes 9:179–187.']
  • ['Lewis CE, S Zona\u20022008\u2002Leucothrinax morrisii, a new name for a familiar Caribbean palm. Palms 52:84–88.']
  • ['Magellan TM, C Husby, S Cuestas, MP Griffith\u20022013\u2002Spent coffee grounds do not control cycad aulacaspis scale. HortTechnology 23:201–206.']
  • ['Marler TE\u20022012\u2002Cycad aulacaspis scale invades the Mariana Islands. Mem N Y Bot Gard 106:20–35.']
  • ['Marler TE, A Moore\u20022010\u2002Cryptic scale infestations on Cycas revoluta facilitate scale invasions. HortScience 45:837–839.']
  • ['Marshall DR, AHD Brown\u20021975\u2002Optimum sampling strategies in genetic conservation. Pages 53–80 in OH Frankel, JG Hawkes, eds. Crop genetic resources for today and tomorrow. Cambridge University Press, Cambridge.']
  • ['Meerow AW, J Francisco-Ortega, M Calonje, MP Griffith, T Ayala-Silva, DW Stevenson, K Nakamura\u20022012\u2002Zamia (Cycadales: Zamiaceae) on Puerto Rico: asymmetric genetic differentiation and the hypothesis of multiple introductions. Am J Bot 99:1828–1839.']
  • ['Meerow AW, K Nakamura\u20022007\u2002Ten microsatellite loci from Zamia integrifolia (Zamiaceae). Mol Ecol Notes 7:824–826.']
  • ['Murphy V, K Moore, MP Griffith, C Husby\u20022013\u2002Improving conservation through cultivation: nine container substrates influence growth of a rare cycad, Zamia pumila L. HortScience 48:1168–1172.']
  • ['Nagalingum NS, CR Marshall, TB Quental, HS Rai, DF Little, S Mathews\u20022011\u2002Recent synchronous radiation of a living fossil. Science 334:796–799.']
  • ['Namoff S, CE Husby, J Francisco-Ortega, LR Noblick, CE Lewis, MP Griffith\u20022010\u2002How well does a botanical garden collection of rare palms capture the genetic variation in a wild population? Biol Conserv 143:1110–1117.']
  • ['Negrón-Ortiz V, DL Gorchov, GJ Breckon\u20021996\u2002Population structure in Zamia (Zamiaceae) in northern Puerto Rico. II. Seed germination and stage-structured population projection. Int J Plant Sci 157:605–614.']
  • ['Nei M\u20021978\u2002Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590.']
  • ['Orloci L\u20021978\u2002Multivariate analysis in vegetation research. 2nd ed. Junk, The Hague.']
  • ['Osborne R\u20021989\u2002Cycad pollen survives ultra-low temperature. Encephalartos 17:25.']
  • ['Osborne R, MA Calonje, KD Hill, L Stanberg, DW Stevenson\u20022012\u2002The world list of cycads. Mem N Y Bot Gard 106:480–507.']
  • ['Peakall R, PE Smouse\u20022006\u2002GENALEX 6: genetic analysis in Excel—population genetic software for teaching and research. Mol Ecol Notes 6:288–295.']
  • ['Pence VC\u20022011\u2002Evaluating costs for the in vitro propagation and preservation of endangered plants. In Vitro Cell Dev Biol Plant 47:176–187.']
  • ['Pritchard WH, A Lindström, N Li, J Nadarajan\u20022011\u2002Integrated conservation of ancient plants (ICAP). J Fairy Lake Bot Gard 10:15.']
  • ['Raimondo DC, JS Donaldson\u20022003\u2002Responses of cycads with different life histories to the impact of plant collecting: simulation models to determine important life history stages and population recovery times. Biol Conserv 111:345–358.']
  • ['Raven P, K Havens\u20022014\u2002Ex situ plant conservation and cryopreservation: breakthroughs in tropical plant conservation. Int J Plant Sci 175:1–2.']
  • ['Rucińska A, J Puchalski\u20022011\u2002Comparative molecular studies on the genetic diversity of an ex situ garden collection and its source population of the critically endangered Polish endemic plant Cochlearia polonica E. Fröhlich. Biodivers Conserv 20:401–413.']
  • ['Salas-Leiva D, AW Meerow, M Calonje, MP Griffith, J Francisco-Ortega, DW Stevenson, K Nakamura, CE Lewis, S Namoff\u20022013\u2002Phylogeny of the cycads based on multiple single-copy nuclear genes: congruence of concatenated parsimony, likelihood and species tree inference methods. Ann Bot 112:1263–1278.']
  • ['Schaal B, WJ Leverich\u20022004\u2002Population genetic issues in ex situ plant conservation. Pages 267–285 in EO Guerrant Jr, K Havens, M Maunder, eds. Ex situ plant conservation: supporting species survival in the wild. Island, Washington, DC.']
  • ['Schemske DW, BC Husband, MH Ruckelshaus, C Goodwillie, IM Parker, JG Bishop\u20021994\u2002Evaluating approaches to the conservation of rare and endangered plants. Ecology 75:584–606.']
  • ['Seeds of Success\u20022012\u2002Bureau of land management technical protocol for the collection, study, and conservation of seeds from native plant species for Seeds of Success.']
  • ['Sharrock S\u20022012\u2002GSPC global strategy for plant conservation: a guide to the GSPC. Botanic Gardens Conservation International, Richmond, UK.']
  • ['Vovides AP, N Ogata, V Sosa, E Pena\u20021997\u2002Pollination of endangered Cuban cycad Microcycas calocoma (Miq.) A.DC. Bot J Linn Soc 25:201–210.']
  • ['Walters TW\u20022003\u2002Off-site collections. Pages 48–53 in J Donaldson, ed. Cycads: status survey and conservation action plan. International Union for Conservation of Nature, Gland, Switzerland.']
  • ['Walters TW, DS Decker-Walters\u20021991\u2002Patterns of allozyme diversity in the West Indian cycad Zamia pumila (Zamiaceae). Am J Bot 78:436–449.']
  • ['Ware GO, RD Phillips, RS Parrish, LC Moon\u20021980\u2002A comparison of two nonlinear models for describing intake-response relationships in higher organisms. J Nutr 110:765–770.']
  • ['Woodenberg W, D Erdey, N Pammenter, P Berjak\u20022007\u2002Post-shedding seed behavior of selected Encephalartos species: abstracts from the 5th International Workshop on Desiccation Tolerance and Sensitivity of Seeds and Vegetative Plant Tissues. S Afr J Bot 73:496.']
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