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Cloning and Analysis of Strong Promoters is Made Possible by the Downstream Placement of a RNA Termination Signal
Reiner Gentz, Annette Langner, Annie C. Y. Chang, Stanley N. Cohen and Hermann Bujard
Proceedings of the National Academy of Sciences of the United States of America
Vol. 78, No. 8, [Part 2: Biological Sciences] (Aug., 1981), pp. 4936-4940
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/10457
Page Count: 5
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Downstream placement of a strong transcriptional termination signal has made possible the cloning of bacteriophage T5 promoters known to exhibit high signal strength. The cloning system constructed contains two easily assayable indicator functions whose expression is controlled by the integration of promoters and terminators, respectively. By assessing transcription within the indicator regions, the efficiency of promoters as well as termination signals can be determined in vitro and in vivo.
Proceedings of the National Academy of Sciences of the United States of America © 1981 National Academy of Sciences