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Expression of the denV Gene of Bacteriophage T4 Cloned in Escherichia coli
R. Stephen Lloyd and Philip C. Hanawalt
Proceedings of the National Academy of Sciences of the United States of America
Vol. 78, No. 5, [Part 2: Biological Sciences] (May, 1981), pp. 2796-2800
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/10705
Page Count: 5
You can always find the topics here!Topics: Bacteriophages, Plasmids, DNA, Bacteriophage T4, Pyrimidine dimers, Dimers, DNA repair, Enzymes, Chemical suspensions, Irradiation
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The denV gene of bacteriophage T4 has been cloned into Escherichia coli K-12 by inserting appropriate fragments of cytosine-containing T4 DNA into the Sal I site of the plasmid pBR322. The denV gene codes for an enzyme that initiates the excision repair of pyrimidine dimers produced in DNA by UV. In uvrA recA mutants, deficient in an early step in excision repair, the cloned DNA results in enhanced UV resistance that is more pronounced in stationary- than in exponential-phase cultures. The expression of the cloned DNA also results in the enhanced survival of UV-irradiated phage λ or of a denV mutant of phage T4 and in removal of dimers from the DNA of UV-irradiated cells.
Proceedings of the National Academy of Sciences of the United States of America © 1981 National Academy of Sciences