Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Physical Linkage of the Constant Region Genes for Immunoglobulins λ I and λ III

Jim Miller, Alfred Bothwell and Ursula Storb
Proceedings of the National Academy of Sciences of the United States of America
Vol. 78, No. 6, [Part 2: Biological Sciences] (Jun., 1981), pp. 3829-3833
Stable URL: http://www.jstor.org/stable/10931
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Physical Linkage of the Constant Region Genes for Immunoglobulins λ I and λ III
Preview not available

Abstract

During differentiation from a stem cell to an antibody-secreting cell, the immunoglobulin genes within a B cell undergo a rearrangement that juxtaposes a variable region gene to a constant region gene. To analyze the genetic organization of an immunoglobulin gene family in nonrearranged, germ-line DNA, we have constructed a recombinant DNA library from randomly cleaved mouse kidney DNA fragments. From this library, we have isolated three overlapping recombinant clones containing the constant region gene for λ I light chains (Cλ I). These clones spanned 24.9 kilobases of mouse DNA and contained no variable region sequences. Hybridization of these clones with λ II cDNA demonstrated the presence of an additional constant region gene and a joining region 3.2 kilobases 5′ of Cλ I. This gene was tentatively identified as Cλ III by the absence of an Ava I endonuclease site, which is present within Cλ II. The Cλ III amino acid sequence has recently been reported [Azuma, T., Steiner, L. A. & Eisen, H. N. (1981) Proc. Natl. Acad. Sci. USA 78, 569-573] and is very closely related to the Cλ II amino acid sequence.

Page Thumbnails

  • Thumbnail: Page 
3829
    3829
  • Thumbnail: Page 
3830
    3830
  • Thumbnail: Page 
3831
    3831
  • Thumbnail: Page 
3832
    3832
  • Thumbnail: Page 
3833
    3833