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Physical Linkage of the Constant Region Genes for Immunoglobulins λ I and λ III
Jim Miller, Alfred Bothwell and Ursula Storb
Proceedings of the National Academy of Sciences of the United States of America
Vol. 78, No. 6, [Part 2: Biological Sciences] (Jun., 1981), pp. 3829-3833
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/10931
Page Count: 5
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During differentiation from a stem cell to an antibody-secreting cell, the immunoglobulin genes within a B cell undergo a rearrangement that juxtaposes a variable region gene to a constant region gene. To analyze the genetic organization of an immunoglobulin gene family in nonrearranged, germ-line DNA, we have constructed a recombinant DNA library from randomly cleaved mouse kidney DNA fragments. From this library, we have isolated three overlapping recombinant clones containing the constant region gene for λ I light chains (Cλ I). These clones spanned 24.9 kilobases of mouse DNA and contained no variable region sequences. Hybridization of these clones with λ II cDNA demonstrated the presence of an additional constant region gene and a joining region 3.2 kilobases 5′ of Cλ I. This gene was tentatively identified as Cλ III by the absence of an Ava I endonuclease site, which is present within Cλ II. The Cλ III amino acid sequence has recently been reported [Azuma, T., Steiner, L. A. & Eisen, H. N. (1981) Proc. Natl. Acad. Sci. USA 78, 569-573] and is very closely related to the Cλ II amino acid sequence.
Proceedings of the National Academy of Sciences of the United States of America © 1981 National Academy of Sciences