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Suppressor Factor from a T Cell Hybrid Inhibits Delayed-Type Hypersensitivity Responses to Azobenzenearsonate
R. Blake Whitaker, Jerry T. Nepom, Man-Sun Sy, Muneo Takaoki, Colette F. Gramm, Ira Fox, Ronald N. Germain, Mitchell J. Nelles, Mark I. Greene and Baruj Benacerraf
Proceedings of the National Academy of Sciences of the United States of America
Vol. 78, No. 10, [Part 2: Biological Sciences] (Oct., 1981), pp. 6441-6445
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/11084
Page Count: 5
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By using polyethylene glycol 1540, BW5147 AKR T lymphoma cells were fused with splenocytes from A/J mice treated so as to induce suppressor T cells specific for azobenzene-arsonate (ABA). Of 576 microwells originally seeded, 132 demonstrated growing cell clones, 4 of which produced an ABA-binding supernatant factor. When tested in vivo for suppression of delayed-type hypersensitivity to ABA, two of these cell lines, A4 and F12, were shown to produce suppressive supernatant factors. Fluorescence analysis of the F12 cells with appropriate antisera demonstrated this T cell hybrid to be Thy 1.2+, Lyt 1+, 2-, and surface immunoglobulin negative, the surface marker phenotype of conventional ABA-specific suppressor T cells. This cloned suppressor cell line, F12, produces a culture supernatant factor that is suppressive at dilutions up to 1:100 and has provided material for genetic and immunochemical analysis.
Proceedings of the National Academy of Sciences of the United States of America © 1981 National Academy of Sciences