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Transposon-Specified Site-Specific Recombination

Paul Kitts, Lorraine Symington, Mary Burke, Randall Reed and David Sherratt
Proceedings of the National Academy of Sciences of the United States of America
Vol. 79, No. 1, [Part 1: Biological Sciences] (Jan. 1, 1982), pp. 46-50
Stable URL: http://www.jstor.org/stable/11130
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Transposon-Specified Site-Specific Recombination
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Abstract

Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element γ δ (Tn1000) synthesizes a product interchangeable with the Tn1/3tnpR protein in promoting Tn1/3 site-specific recombination. These data support the hypothesis that cointegrates containing directly repeated copies of Tn1/3 are obligatory intermediates in interreplicon transposition of Tn1/3. In addition, we show here that the reaction is independent of the element-encoded tnpA gene product. Tn501, which specifies mercury resistance, also produces cointegrates as intermediates in interreplicon transposition. The appearance of Tn501-specified recombination activity that can act on these cointegrates requires growth of cells in the presence of Hg2+.

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