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Na and K Dependence of the Na/K Pump in Cystic Fibrosis Fibroblasts
Vivian M. Reznik, Jerry A. Schneider and Stanley A. Mendoza
Proceedings of the National Academy of Sciences of the United States of America
Vol. 78, No. 11, [Part 2: Biological Sciences] (Nov., 1981), pp. 7143-7146
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/11426
Page Count: 4
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The Na and K dependence of the Na/K pump was measured in skin fibroblasts from patients with cystic fibrosis and age/sex-matched controls. Under basal conditions, there was no difference between control and cystic fibrosis cells in protein per cell, intracellular Na and K content, or Na/K pump activity (measured as ouabain-sensitive 86Rb uptake). There was no difference in the Na dependence of the Na/K pump between cystic fibrosis cells and control cells. In cells from patients with cystic fibrosis, the Na/K pump had a significantly lower affinity for K (Km = 1.6 mM) when compared to normals (Km = 0.9 mM). This difference was demonstrated by using two independent experimental designs.
Proceedings of the National Academy of Sciences of the United States of America © 1981 National Academy of Sciences