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Establishment of a Cell Line from Lepidopteran Wing Imaginal Discs: Induction of Newly Synthesized Proteins by 20-hydroxyecdysone
D. E. Lynn, S. G. Miller and H. Oberlander
Proceedings of the National Academy of Sciences of the United States of America
Vol. 79, No. 8, [Part 1: Biological Sciences] (Apr. 15, 1982), pp. 2589-2593
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/12182
Page Count: 5
You can always find the topics here!Topics: Cell lines, Cells, Cell culture techniques, Imaginal discs, Cultured cells, Insect hormones, Insect development, Gels, Tissue culture techniques, Fat body
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A cell line has been developed from the imaginal wing discs of the lepidopteran insect Trichoplusia ni (Hübner). The cells grow in suspension as fluid-filled, multicellular vesicles. The cell line has been designated IAL-TND1. Two-dimensional electrophoresis (isoelectric focusing and sodium dodecyl sulfate/polyacrylamide gels) of [35 S]methionine-labeled proteins revealed similar patterns for IAL-TND1 cells and whole T. ni wing imaginal discs. Additionally, exposure to the insect steroid hormone 20-hydroxyecdysone stimulated the synthesis of the same new proteins in both whole wing discs and IAL-TND1 cell vesicles.
Proceedings of the National Academy of Sciences of the United States of America © 1982 National Academy of Sciences