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Saturation of, and Competition for Entry into, the Apical Secretory Pathway
Alan D. Marmorstein, Karl G. Csaky, Judit Baffi, Linda Lam, Firas Rahaal and Enrique Rodriguez-Boulan
Proceedings of the National Academy of Sciences of the United States of America
Vol. 97, No. 7 (Mar. 28, 2000), pp. 3248-3253
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/121877
Page Count: 6
You can always find the topics here!Topics: Secretion, Cells, Adenoviruses, Receptors, Secretory pathway, Epithelial cells, Charge separation, Antibodies, Enzyme linked immunosorbent assay, Molecules
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To investigate mechanisms of apical sorting in the secretory pathway of epithelial cells, we expressed varying amounts of the 165 amino acid isoform of vascular endothelial growth factor (VEGF165) and transforming growth factor β 1(TGF-β 1) via replication defective adenoviruses. Apical sorting of both proteins was efficient at low expression levels but saturated or was reversed at high expression levels. High expression levels of TGF-β 1 were effective at competing VEGF165 out of the apical pathway; however, VEGF165 did not compete out TGF-β 1. Tunicamycin inhibition experiments showed that the apical polarity of VEGF165 was independent of N-glycosylation. We conclude that the apical sorting of these two molecules is a saturable, signal-mediated process, involving competition for apical sorting receptors. The sorting of the two proteins does not appear to involve N-glycans as sorting signals, or lectin sorters. The observations are particularly relevant to gene therapy because they demonstrate that overexpression of a transgene can result in undesirable missorting of the encoded protein.
Proceedings of the National Academy of Sciences of the United States of America © 2000 National Academy of Sciences