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An Improved Method of DNA Isolation from Plants Collected in the Field and Conserved in Saturated NaCl/CTAB Solution
Helena Štorchová, Radmila Hrdličková, Jindřich Chrtek, Jr., Martin Tetera, Dorothee Fitze and Judith Fehrer
Vol. 49, No. 1 (Feb., 2000), pp. 79-84
Published by: International Association for Plant Taxonomy (IAPT)
Stable URL: http://www.jstor.org/stable/1223934
Page Count: 6
You can always find the topics here!Topics: Leaves, Polymerase chain reaction, Plants, Silica gel, Genomics, Materials preservation, Plant products, Grants, Taxa, Precipitation
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A simple method for isolation of genomic DNA from wild plants sampled in remote field areas is presented. The protocol combines NaCl/CTAB leaf preservation with sorbitol extraction of secondary compounds which often contain inhibitors of Taq DNA polymerase activity. The obtained DNA is suitable for random amplified polymorphic DNA (RAPD) analysis of plant populations as well as for specific amplification of chloroplast DNA sequences. The NaCl/CTAB leaf preservation is a powerful alternative to silica gel drying-based preservation.
Taxon © 2000 International Association for Plant Taxonomy (IAPT)