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An Improved Method of DNA Isolation from Plants Collected in the Field and Conserved in Saturated NaCl/CTAB Solution

Helena Štorchová, Radmila Hrdličková, Jindřich Chrtek, Jr., Martin Tetera, Dorothee Fitze and Judith Fehrer
Taxon
Vol. 49, No. 1 (Feb., 2000), pp. 79-84
DOI: 10.2307/1223934
Stable URL: http://www.jstor.org/stable/1223934
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
An Improved Method of DNA Isolation from Plants Collected in the Field and Conserved in Saturated NaCl/CTAB Solution
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Abstract

A simple method for isolation of genomic DNA from wild plants sampled in remote field areas is presented. The protocol combines NaCl/CTAB leaf preservation with sorbitol extraction of secondary compounds which often contain inhibitors of Taq DNA polymerase activity. The obtained DNA is suitable for random amplified polymorphic DNA (RAPD) analysis of plant populations as well as for specific amplification of chloroplast DNA sequences. The NaCl/CTAB leaf preservation is a powerful alternative to silica gel drying-based preservation.

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