Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Poly(ADP-Ribosyl)ation of Polynucleosomes Causes Relaxation of Chromatin Structure

Guy G. Poirier, Gilbert De Murcia, Jenny Jongstra-Bilen, Claude Niedergang and Paul Mandel
Proceedings of the National Academy of Sciences of the United States of America
Vol. 79, No. 11, [Part 1: Biological Sciences] (Jun. 1, 1982), pp. 3423-3427
Stable URL: http://www.jstor.org/stable/12294
Page Count: 5
  • Read Online (Free)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Poly(ADP-Ribosyl)ation of Polynucleosomes Causes Relaxation of Chromatin Structure
Preview not available

Abstract

When rat pancreatic polynucleosomes were poly(ADP-ribosyl)ated with purified calf thymus poly(ADP-ribose) polymerase and examined by electron microscopy, a relaxation of their native zigzag structure was observed. At high ionic strengths control nucleosomes condensed into 250- angstrom -thick fibers, but poly(ADP-ribosyl)ated polynucleosomes did not; they showed a close resemblance to chromatin depleted of histone H1. The relaxed state of poly(ADP-ribosyl)ated polynucleosomes was also confirmed by sedimentation velocity analysis. Histone H1 was found to be the major histone acceptor of poly(ADP-ribose). Poly(ADP-ribose) linked to histone H1 did not seem to cause its dissociation from the chromatin, but it impaired significantly its effect on chromatin condensation.

Page Thumbnails

  • Thumbnail: Page 
3423
    3423
  • Thumbnail: Page 
3424
    3424
  • Thumbnail: Page 
3425
    3425
  • Thumbnail: Page 
3426
    3426
  • Thumbnail: Page 
3427
    3427