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A Conserved Mechanism of Retrovirus Restriction in Mammals
Greg Towers, Michael Bock, Samia Martin, Yasuhiro Takeuchi, Jonathan P. Stoye and Olivier Danos
Proceedings of the National Academy of Sciences of the United States of America
Vol. 97, No. 22 (Oct. 24, 2000), pp. 12295-12299
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/123831
Page Count: 5
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The murine Fv1 gene restricts infection by N- or B-tropic murine leukemia viruses at a postentry, preintegration stage. The Fv1-sensitive viruses previously used for the study of Fv1 encode an ecotropic envelope gene and thus only infect rodent cells. Consequently, the study of Fv1 restriction has been carried out solely in mice and murine cell lines. By infection with retroviral vectors containing N- or B-tropic core and pantropic vesicular stomatitis virus-G envelope protein, we now demonstrate that cell lines derived from various mammalian species, including humans, have an Fv1-like retrovirus restriction function, preventing N-tropic vector infection. Like Fv1, restriction is directed at amino acid 110 of the viral capsid protein. In contrast to Fv1, the novel restriction is characterized by the absence of reverse-transcribed viral DNA. We speculate that these activities have been selected for by retroviral epidemics in the distant past.
Proceedings of the National Academy of Sciences of the United States of America © 2000 National Academy of Sciences