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Encapsidation Sequences for Spleen Necrosis Virus, an Avian Retrovirus, are between the 5 Long Terminal Repeat and the Start of the Gag Gene

Shinichi Watanabe and Howard M. Temin
Proceedings of the National Academy of Sciences of the United States of America
Vol. 79, No. 19, [Part 1: Biological Sciences] (Oct. 1, 1982), pp. 5986-5990
Stable URL: http://www.jstor.org/stable/12935
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Encapsidation Sequences for Spleen Necrosis Virus, an Avian Retrovirus, are between the 5′ Long Terminal Repeat and the Start of the Gag Gene
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Abstract

The minimal cis-acting sequences outside the long terminal repeat (LTR) required for formation of an infectious retrovirus cloning vector were determined with recombinants of spleen necrosis virus (SNV) DNA and herpes simplex virus type 1 thymidine kinase gene. The 3′ end of SNV DNA was removed to within 40 base pairs (bp) from the 3′ LTR with only a 2-fold effect on the recovery of infectious recombinant virus. However, when the 5′ end of SNV DNA was removed to within 100 bp from the 5′ LTR, infectious recombinant virus was not recovered. Deletion mutants constructed around this latter region showed that nucleotides between 100 and 285 bp from the 5′ LTR are necessary for encapsidation of genomic viral RNA. We call this region required for encapsidation E.

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