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Monoclonal Antibodies Recognize Individual Neurofilament Triplet Proteins

V. Lee, H. L. Wu and W. W. Schlaepfer
Proceedings of the National Academy of Sciences of the United States of America
Vol. 79, No. 19, [Part 1: Biological Sciences] (Oct. 1, 1982), pp. 6089-6092
Stable URL: http://www.jstor.org/stable/12957
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Monoclonal Antibodies Recognize Individual Neurofilament Triplet Proteins
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Abstract

A series of 12 monoclonal antibodies has been prepared against crude or purified individual bovine neurofilament (NF) triplet proteins (designated as P68, P150, and P200). Six of the antibodies recognized unique determinants on individual NF subunits. The other six recognized shared determinants on at least two NF triplet proteins. Six of the antibodies recognized separated, immobilized individual NF in nitrocellulose paper. By the peroxidase-antiperoxidase technique, all of the antibodies stained neurons and their processes in a manner identical to the wellknown distribution of NF in both the central and peripheral nervous system. These results strongly suggest that the NF subunits are distinct but related proteins and that the smaller NF subunits (P68 and P150) are not derived from larger NF proteins.

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