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Three Distinct Antigens Associated with Human T-Lymphocyte-Mediated Cytolysis: LFA-1, LFA-2, and LFA-3
Francisco Sanchez-Madrid, Alan M. Krensky, Carl F. Ware, Elizabeth Robbins, Jack L. Strominger, Steven J. Burakoff and Timothy A. Springer
Proceedings of the National Academy of Sciences of the United States of America
Vol. 79, No. 23, [Part 1: Biological Sciences] (Dec. 1, 1982), pp. 7489-7493
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/13113
Page Count: 5
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Monoclonal antibodies were prepared to anti-HLA-DR cytolytic T lymphocytes (CTLs) and screened for inhibition of CTL-mediated killing. Binding of monoclonal antibodies to four types of molecules, LFA-1, LFA-2, LFA-3, and HLA-DR, inhibited killing, suggesting that these molecules participate in the CTL-target cell interaction. The antigens were characterized by immunoprecipitation, crosslinking, NaDodSO4/polyacrylamide gel electrophoresis, and immunofluorescence flow cytometry. The LFA-1 antigen contains α and β polypeptide chains of Mr 177,000 and 95,000 that are noncovalently associated in an α 1β 1 structure. It is present on both B and T lymphocytes and marks subpopulations that differ in quantitative expression. Human LFA-1 appears to be the homologue of mouse LFA-1. Human LFA-2 is of Mr 49,000 with a minor component of Mr 36,000. It is expressed on CTL lines but not on a B-cell line and in peripheral blood preferentially on T lymphocytes. Human LFA-3 is of Mr 60,000 and is expressed on both B and T lymphocytes.
Proceedings of the National Academy of Sciences of the United States of America © 1982 National Academy of Sciences