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Synthesizing Enzymes for Four Neuroactive Substances in Motor Neurons and Neuromuscular Junctions: Light and Electron Microscopic Immunocytochemistry
Victoria Chan-Palay, Andrew G. Engel, Sanford L. Palay and Jang-Yen Wu
Proceedings of the National Academy of Sciences of the United States of America
Vol. 79, No. 21, [Part 1: Biological Sciences] (Nov. 1, 1982), pp. 6717-6721
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/13299
Page Count: 5
You can always find the topics here!Topics: Antibodies, Enzymes, End plates, Motor neurons, Synapses, Nerves, Sulfinic acids, Cryostats, Spinal cord, Neurons
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Immunocytochemical evidence is presented for the existence of choline acetyltransferase (ChoAcTase), cysteine sulfinic acid decarboxylase (CSADCase), tyrosine hydroxylase (TyrOHase), and glutamic acid decarboxylase (GluDCase) in large motor neurons of the hypoglossal nucleus and the spinal cord and in nerve terminals of motor end plates in tongue and skeletal muscle of five mammalian species, including man. These enzymes, which are responsible for the synthesis of acetylcholine (AcCho), taurine, dopamine, and γ -aminobutyrate (GABA), respectively, were detected by immunocytochemical studies with monoclonal or polyclonal antibodies raised against the enzymes. Electron microscopy of the neuromuscular junctions showed that the immunoreactivity in each case was confined to the cytoplasmic matrix of presynaptic nerve terminals. Immunoreactivity obtained for each enzyme antibody varied with the species. It was highest in fresh, unfixed muscle and lowest in aldehyde-fixed specimens. Negative controls were obtained with preimmune sera and antisera preabsorbed with pure ChoAcTase, CSADCase, or GluDCase antigen. Double-labeling studies with ChoAcTase antibodies and acetylcholinesterase (AcChoEase) antibodies, AcChoEase enzyme activity, or α -bungarotoxin binding indicated that ChoAcTase, AcChoEase, and AcCho receptors were colocalized at the same end plates.
Proceedings of the National Academy of Sciences of the United States of America © 1982 National Academy of Sciences