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Correct Transcription of a Cloned Mouse Immunoglobulin Gene in vivo

Didier Picard and Walter Schaffner
Proceedings of the National Academy of Sciences of the United States of America
Vol. 80, No. 2, [Part 1: Biological Sciences] (Jan. 15, 1983), pp. 417-421
Stable URL: http://www.jstor.org/stable/13619
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Correct Transcription of a Cloned Mouse Immunoglobulin Gene in vivo
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Abstract

We have obtained correct transcripts from the mouse immunoglobulin λ I light chain gene on transfection into human HeLa cells. Linkage to simian virus 40 (SV40) DNA containing a transcriptional ``enhancer'' element was required to raise λ -chain gene transcription to a detectable level in our transient-expression assay. The transcripts had the same 3′ end as authentic λ I mRNA when the SV40 enhancer element was 150 base pairs upstream from the cap site. The situation was different when the λ -chain gene promoter was separated from the SV40 sequences by more than 1 kilobase pair of spacer DNA; then λ -chain gene transcripts were not correctly initiated in human HeLa, monkey CV-1, and mouse 3T6 cells. In this respect, the λ -chain gene behaves differently from the rabbit β -globin gene, which can be activated by the SV40 enhancer over distances of several kilobases [Banerji, J., Rusconi, S. & Schaffner, W. (1981) Cell 27, 299-308].

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