Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If you need an accessible version of this item please contact JSTOR User Support

A Reliable Molecular Method of Gender Determination for Mammals

Carla N. Shaw, Paul J. Wilson and Bradley N. White
Journal of Mammalogy
Vol. 84, No. 1 (Feb., 2003), pp. 123-128
Stable URL: http://www.jstor.org/stable/1383640
Page Count: 6
  • Read Online (Free)
  • Download ($42.00)
  • Cite this Item
If you need an accessible version of this item please contact JSTOR User Support
A Reliable Molecular Method of Gender Determination for Mammals
Preview not available

Abstract

A polymerase chain reaction-based method was developed for gender determination in a wide variety of marine and terrestrial mammals, including cetaceans, pinnipeds, ungulates, canids, and ursids. An intron within the zinc-finger x (Zfx) and zinc-finger y (Zfy) genes was amplified. Size variation between the Zfx and Zfy introns results in a Y-specific band in males. Size of the Zfy intron varies more across species than that of the Zfx intron. This technique is simple and rapid, requires small amounts of DNA, and can be applied to a variety of mammalian species. In contrast to previous methods, only 1 primer set is needed for both gender determination and internal verification of amplification.

Page Thumbnails

  • Thumbnail: Page 
123
    123
  • Thumbnail: Page 
124
    124
  • Thumbnail: Page 
125
    125
  • Thumbnail: Page 
126
    126
  • Thumbnail: Page 
127
    127
  • Thumbnail: Page 
128
    128