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Immune Response of Snakes

Sylvia Kendall Salanitro and Sherman A. Minton, Jr.
Copeia
Vol. 1973, No. 3 (Aug. 28, 1973), pp. 504-515
DOI: 10.2307/1443116
Stable URL: http://www.jstor.org/stable/1443116
Page Count: 12
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Immune Response of Snakes
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Abstract

Colubrid snakes were immunized with bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH) in Freund's adjuvant. Specific antibody was first detected four weeks after primary immunization, and the titer remained constant for a period of three months. Two weeks after secondary immunization with either antigen, increased antibody activity, which persisted four months, was noted. Evidence for immunological memory is based on a shorter induction period and a higher antibody level following secondary immunization with KLH. A component isolated from immunized snake serum was identified as an immunoglobulin on the basis of antibody activity and electrophoretic mobility. Sucrose density gradient centrifugation of radioactively labelled snake globulin along with a marker protein, rabbit gamma globulin (6.5S), established a sedimentation coefficient of 7.0S and 7.1S for snake globulin. Heavy chains of both globulins chromatographed together and light chains eluted in the same volume further establishing that the structure of the snake globulin molecule is similar to that of rabbit gamma globulin. Sedimentation coefficient values, determined by sucrose density gradient centrifugation of whole snake serum were approximately 7S for antibody to BSA and 19S for KLH antibody. Evidently there are at least two classes of immunoglobulins in snakes.

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