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Fluorescence-Activated Cell Sorting of Mouse-Human Hybrid Cells Aids in Locating the Gene for the Leu 7 (HNK-1) Antigen to Human Chromosome 11
Jim Schroder, Barbro Nikinmaa, Paula Kavathas and Leonard A. Herzenberg
Proceedings of the National Academy of Sciences of the United States of America
Vol. 80, No. 11, [Part 1: Biological Sciences] (Jun. 1, 1983), pp. 3421-3424
Published by: National Academy of Sciences
Stable URL: http://www.jstor.org/stable/14546
Page Count: 4
You can always find the topics here!Topics: Chromosomes, Amino acids, Human chromosomes, Genes, Antigens, Natural killer cells, Hybridity, Somatic cells, Lymphocytes, Surface antigens
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Leu 7 (HNK-1) is a membrane antigen expressed on human natural killer cells and some other lymphoid cells. Starting with two clones of mouse-human hybrid lymphoid cells that had 1.6% and 35% Leu 7-positive cells, respectively, we viably sorted Leu 7-positive and -negative cells using a fluorescence-activated cell sorter (FACS). Short-term progeny of the sorted cells were then karyotyped. Chromosome 11 was the only human chromosome that was absent from the Leu 7-negative population and present in nearly all of the progeny of the Leu 7-positive selected cells. Thus, we assigned the Leu 7 gene to chromosome 11.
Proceedings of the National Academy of Sciences of the United States of America © 1983 National Academy of Sciences