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SEM and Light Microscope Mounting and Specimen Location Technique for Same-Specimen Study of Palynological Strew Mounts

Pierre A. Zippi
Micropaleontology
Vol. 37, No. 4 (1991), pp. 407-413
DOI: 10.2307/1485913
Stable URL: http://www.jstor.org/stable/1485913
Page Count: 7
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SEM and Light Microscope Mounting and Specimen Location Technique for Same-Specimen Study of Palynological Strew Mounts
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Abstract

A reliable and efficient technique has been developed for the mounting, location and quick re-location of palynological specimens in strew mounts for SEM (scanning electron microscope) and subsequent LM (light microscope) examination. Specimen locations are recorded with reference to a numbered location grid, which has been inked onto a glass coverslip and is visible, both under the SEM and in light. After SEM examination, a permanent LM slide can be made by removing the coverslip from the aluminum stub and inverting it into a non-aqueous mounting medium. Specimens can be quickly re-located under the SEM or the light microscope by referring to grid map records. With this method, both surface (SEM) and internal (LM) features of palynomorphs are readily available for the accurate description and identification of specimens. The resolution of gold coated specimens in LM compares favorably with conventionally mounted specimens.

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