You are not currently logged in.
Access JSTOR through your library or other institution:
If You Use a Screen ReaderThis content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Continuous Imaging of Amino-Acid Translocation in Intact Mycelia of Phanerochaete velutina Reveals Rapid, Pulsatile Fluxes
M. Tlalka, S. C. Watkinson, P. R. Darrah and M. D. Fricker
The New Phytologist
Vol. 153, No. 1 (Jan., 2002), pp. 173-184
Stable URL: http://www.jstor.org/stable/1513919
Page Count: 12
You can always find the topics here!Topics: Mycelium, Signals, Fungi, Inoculum, Photons, Imaging, Scintillation, Phanerochaete, Amino acids, Hyphae
Were these topics helpful?See something inaccurate? Let us know!
Select the topics that are inaccurate.
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Preview not available
• Nitrogen translocation by woodland fungi is ecologically important, however, techniques to study long-distance amino-acid transport in mycelia currently have limited spatial and temporal resolution. We report a new continuous, noninvasive imaging technique for β-emitters that operates with submillimetre spatial resolution and a practical sampling interval of 10-60 min. • Transport of the nonmetabolized, 14 C-labelled amino-acid analogue, α-aminoisobutyric acid (AIB) was imaged using a photon-counting camera as it was transported in foraging mycelium of the cord-forming woodland fungus, Phanerochaete velutina, grown over an intensifying screen in microcosms. • The maximum acropetal transport velocity of 14 C- AIB to the colony margin was 50 mm h-1 (average 23 mm h-1), with a mass transfer of 4.6-51.5 pmol 14 C- AIB h-1 per cord. Transport in cords had a pulsatile component with a period of 11-12 h. • Transport was significantly faster than diffusion, consistent with rapid cycling of nutrients throughout the mycelium between loading and sink regions. The increased spatial and temporal resolution of this method also revealed the rhythmic nature of transport in this fungus for the first time.
The New Phytologist © 2002 New Phytologist Trust