Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If you need an accessible version of this item please contact JSTOR User Support

The Use of the Mosquito Bioassay for Determining the Toxicity to Man of Ciguateric Fish

R. Bagnis, M. Barsinas, C. Prieur, A. Pompon, E. Chungue and A. M. Legrand
Biological Bulletin
Vol. 172, No. 1 (Feb., 1987), pp. 137-143
DOI: 10.2307/1541614
Stable URL: http://www.jstor.org/stable/1541614
Page Count: 7
  • More info
  • Cite this Item
If you need an accessible version of this item please contact JSTOR User Support
The Use of the Mosquito Bioassay for Determining the Toxicity to Man of Ciguateric Fish
Preview not available

Abstract

Moderately well-correlated with the mouse bioassay, requiring a much smaller amount of raw fish flesh, and employing a faster chemical extraction procedure, the mosquito bioassay represents significant progress in detecting ciguatoxin in fish muscle. We use the mosquito bioassay to evaluate the approximate amount of ciguatoxin present in the remains of 25 fish of 17 different species involved in 99 documented cases of ciguatera poisoning. From the data provided by mosquito bioassay and human symptomatology, we determine a dose-effect relationship for ciguatoxin (CTX) to man. Three average pathogenic doses (PD) are established. The minimum PD is estimated to be 0.6 ng CTX/kg, the PD50 to be 2 ng CTX/kg, and the PD100 to be 8 ng CTX/kg. These data suggest a MLD (minimal lethal dose) value of 20 ng CTX/kg.

Page Thumbnails

  • Thumbnail: Page 
137
    137
  • Thumbnail: Page 
138
    138
  • Thumbnail: Page 
139
    139
  • Thumbnail: Page 
140
    140
  • Thumbnail: Page 
141
    141
  • Thumbnail: Page 
142
    142
  • Thumbnail: Page 
143
    143