If you need an accessible version of this item please contact JSTOR User Support

Antibacterial Properties of Isolated Amoebocytes from the Sea Anemone Actinia equina

Danielle M. C. Hutton and Valerie J. Smith
Biological Bulletin
Vol. 191, No. 3 (Dec., 1996), pp. 441-451
DOI: 10.2307/1543017
Stable URL: http://www.jstor.org/stable/1543017
Page Count: 11
  • Download PDF
  • Cite this Item

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If you need an accessible version of this item please contact JSTOR User Support
Antibacterial Properties of Isolated Amoebocytes from the Sea Anemone Actinia equina
Preview not available

Abstract

The antimicrobial defenses of anthozoans were investigated in vitro by extracting amoebocytes from the mesenteric filaments of the beadlet anemone, Actinia equina, and testing for their ability to phagocytose and kill the gram-negative bacterium Psychrobacter immobilis. Only the hyaline amoebocytes exhibited phagocytosis in vitro, with about 40% seen to ingest one or more bacteria over 45 min. Mixed cultures of viable amoebocytes were further found to produce O2- ions and other reactive oxygen species (ROS) after stimulation with phorbol myristate acetate or lipopolysaccharide. Co-incubation of viable amoebocytes with P. immobilis for 3 h in vitro resulted in reduced growth of the bacterium compared to saline-incubated bacteria, but because the growth of P. immobilis was also impaired by lysed control amoebocytes, the contribution made to bacterial killing by ROS could not be evaluated. Instead, as confirmed by additional experiments using lysate supernatants of the amoebocytes, it appears that the cells contain soluble bactericidal factors. The nature of these agents is at present unknown, although preliminary tests indicate that killing is not mediated by lysozyme.

Page Thumbnails

  • Thumbnail: Page 
441
    441
  • Thumbnail: Page 
442
    442
  • Thumbnail: Page 
443
    443
  • Thumbnail: Page 
444
    444
  • Thumbnail: Page 
445
    445
  • Thumbnail: Page 
446
    446
  • Thumbnail: Page 
447
    447
  • Thumbnail: Page 
448
    448
  • Thumbnail: Page 
449
    449
  • Thumbnail: Page 
450
    450
  • Thumbnail: Page 
451
    451