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Postecdysial Changes in the Protein and Glycoprotein Composition of the Cuticle of the Blue Crab Callinectes sapidus
Thomas H. Shafer, Robert D. Roer, Charles G. Miller and Richard M. Dillaman
Journal of Crustacean Biology
Vol. 14, No. 2 (May, 1994), pp. 210-219
Published by: on behalf of The Crustacean Society
Stable URL: http://www.jstor.org/stable/1548901
Page Count: 10
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Postecdysial modifications of the cuticle of Callinectes sapidus were evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of soluble protein extracts. Gels stained with Coomassie blue and with silver demonstrated that major changes in protein composition or mobility occurred immediately after ecdysis. Electroblots of SDS-PAGE gels were probed with various biotinylated lectins. The patterns of lectin binding were also markedly different between blots of pre- and postecdysial cuticle extracts, demonstrating a change in glycosylation or glycoprotein mobility in the early postecdysial period. The dramatic alteration in cuticular composition is concurrent with the onset of sclerotization and mineralization of the preexuvially deposited layers of the cuticle.
Journal of Crustacean Biology © 1994 Brill