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Preparation of Mycoplasma Synoviae Hemagglutinating Antigen and Its Use in the Hemagglutination-Inhibition Test
Thomas H. Vardaman and Harry W. Yoder, Jr.
Vol. 13, No. 3 (Aug., 1969), pp. 654-661
Published by: American Association of Avian Pathologists
Stable URL: http://www.jstor.org/stable/1588539
Page Count: 8
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A satisfactory hemagglutinating (HA) antigen was produced from isolate WVU 1853 of M. synoviae grown in a mycoplasma broth formulated by Frey. The HA antigen, employed in the HI test, showed good specificity in differentiating M. synoviae from M. gallisepticum infection. There was no drop in HA titer when the antigen was stored at -65 C for 4 months. When the antigen was stored at -13 C or higher, the drop in HA titer increased as the length of time in storage increased. When 4 units of antigen were used per tube in the HI test, the HI results closely agreed with those of the original antigen stored at -65 C, which had no loss in HA titer.
Avian Diseases © 1969 American Association of Avian Pathologists