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Studies on Avian Heterophils I. Cell Separation
Ralph C. Topp and Herbert C. Carlson
Vol. 16, No. 2 (Jan. - Mar., 1972), pp. 364-368
Published by: American Association of Avian Pathologists
Stable URL: http://www.jstor.org/stable/1588801
Page Count: 5
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A technique devised for separating heterophils from whole turkey blood uses initial low-speed centrifugation, incubation of the blood in a commercial laboratory column packed with siliconized glass beads, and elution of the cells from the column with an EDTA buffer. The technique gave separations containing an average of 74.8% (range 58-94%) heterophils of the total leucocyte population, 90% of which were viable.
Avian Diseases © 1972 American Association of Avian Pathologists