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Identification of Infectious Bronchitis Virus by Interference with the B-1 Isolant of Newcastle Disease Virus. Waxing and Waning of Interference
L. G. Raggi and P. Pignattelli
Vol. 19, No. 2 (Apr. - Jun., 1975), pp. 334-342
Published by: American Association of Avian Pathologists
Stable URL: http://www.jstor.org/stable/1588988
Page Count: 9
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The Massachusetts and the Connecticut types of infectious bronchitis virus (IBV) were identified by interference in embryonating chicken eggs (ECE) with the production of hemagglutinin by the B-1 isolant of Newcastle disease virus (NDV). This interference test appears to be specific because the above interference was eliminated by adding type-specific anti-IBV serum to the IBV-NDV system; however, interference was not detectable when fowlpox virus (FPV) and infectious laryngotracheitis virus (LTV) were substituted for IBV. Specificity of the interference test was dependent upon a system involving IBV, NDV, FPV, and LTV. The test can be done in 3 days and requires minimum laboratory facilities. Most of the experiments were done with the Massachusetts type of IBV. Only a few were with the Connecticut type. The interfering action of the above two types of IBV over the B-1 isolant of NDV waxed between the 24th and 54th hr after inoculation of NDV; it was waning at the 54th-60th hr postinoculation and was undetectable by the 66th-72nd hr.
Avian Diseases © 1975 American Association of Avian Pathologists