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Journal Article

An Enzyme-Linked Immunosorbent Assay for Detecting Avian Leukosis-Sarcoma Viruses

E. J. Smith, A. Fadly and W. Okazaki
Avian Diseases
Vol. 23, No. 3 (Jul. - Sep., 1979), pp. 698-707
DOI: 10.2307/1589746
Stable URL: http://www.jstor.org/stable/1589746
Page Count: 10
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An Enzyme-Linked Immunosorbent Assay for Detecting Avian Leukosis-Sarcoma Viruses
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Abstract

Immunoglobulins from antiserum raised against chromatographically purified avian myeloblastosis virus (AMV) group-specific (gs) antigens were used in an enzyme-linked immunosorbent assay (ELISA). Readily discernible color was produced with 2-3 ng of AMV protein in microplate wells coated with 4 μg of salt-precipitated immunoglobulins. When a biological assay, i.e., phenotypic mixing (PM), was the criterion for the infectious status of specimens, the ELISA consistently identified a greater percentage of virus-positive specimens than direct complement-fixation (DCF) tests. Over 95% concordance was obtained between the ELISA and PM bioassays when meconia and whole-blood samples were tested. Moreover, three DCF(-) egg albumens from one virus shedder hen were positive by the direct ELISA. Complete agreement was found between a biological assay for endogenous virus and the ELISA when blood and albumens from inbred chickens were tested. The ELISA is a rapid and convenient alternative to the DCF test for identifying infected chickens in eradication programs, because virus-rich sources such as meconia and blood that are unsuitable for DCF can be tested directly.

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