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In vivo Antigen Expression by Pasteurella multocida

John R. Glisson and I-Hsin Ning Cheng
Avian Diseases
Vol. 35, No. 2 (Apr. - Jun., 1991), pp. 392-396
DOI: 10.2307/1591196
Stable URL: http://www.jstor.org/stable/1591196
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
In vivo Antigen Expression by Pasteurella multocida
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Abstract

Pasteurella multocida was purified from the blood of turkeys affected with acute fowl cholera, and membrane preparations from those bacteria were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized on immunoblots. Antigens were detected in the membranes of these in vivo-propagated bacteria that were not detected in membrane preparations of the same P. multocida strain grown in vitro. The unique antigens were detected in the detergent-insoluble phase and were enriched to various degrees by different detergents. /// Se purificó la Pasteurella multocida aislada a partir de la sangre de pavos afectados en forma aguda por cólera aviar. Las preparaciones de las membranas de las bacterias fueron separadas mediante electroforesis en un gel de poliacrilamida y dodecil sulfato de sodio, para ser visualizados mediante inmunotransferencia puntual. En las membranas de las bacterias propagadas in vivo se detectaron antígenos no encontrados en las preparaciones de las membranas de bacterias de la misma cepa cultivadas in vitro. Estos antígenos exclusivos de las bacterias cultivadas in vivo fueron detectados en la fase insoluble en detergente y fueron enriquecidos en diversas proporciones por los diferentes detergentes.

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