Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Early Pathogenesis in Chicks of Infection with an Enterotropic Strain of Infectious Bronchitis Virus

A. G. Ambali and R. C. Jones
Avian Diseases
Vol. 34, No. 4 (Oct. - Dec., 1990), pp. 809-817
DOI: 10.2307/1591367
Stable URL: http://www.jstor.org/stable/1591367
Page Count: 9
  • Read Online (Free)
  • Download ($12.00)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Early Pathogenesis in Chicks of Infection with an Enterotropic Strain of Infectious Bronchitis Virus
Preview not available

Abstract

One-day-old specific-pathogen-free chicks were inoculated intranasally and intraocularly with infectious bronchitis virus (strain G). At days 1, 3, 5, 7, 10, and 14 postinfection, three birds were euthanatized, and the virus contents of both enteric tissues and some non-enteric tissues were assayed. Immunofluorescence and histopathological studies were also conducted. Six of 30 chicks died of nephritis between days 5-10 postinfection. Gross kidney lesions were the major pathological abnormalities. Inflammation was observed histologically in trachea, kidney, and rectum. High virus titers were found at various times in trachea, kidney, and all entric tissues except for the jejunum. Relatively high titers of virus were still detectable at day 14 postinfection in the kidney, proventriculus, cecal tonsil, ileum, rectum, and bursa of Fabricius. Immunofluorescence staining showed viral antigens in enterocytes at the tips of villi in the ileum and rectum, and in the bursa. Viral antigens were also demonstrated in the epithelial cells of the trachea and in kidney tubules. /// Se inocularon intranasal e intraocularmente pollitos libres de patógenos específicos de un día de edad con la cepa G del virus de la bronquitis infecciosa. Tres aves fueron sacrificadas en los días 1, 3, 5, 7, 10 y 14 postinfección y se investigó el contenido viral de los tejidos intestinales y no entéricos. También se efectuaron estudios histopatológicos y de inmunofluorescencia. Seis de los 30 pollitos murieron de nefritis entre los días 5 y 10 postinfección. Las lesiones macroscópicas del riñón fueron las principales anormalidades patológicas. Histológicamente, se observó inflamación de la tráquea, riñón y recto. Con excepción del yeyuno, se encontraron altos títulos virales en la tráquea, riñón y todos los tejidos entéricos en distintos momentos. En el día 14 postinfección todavía eran detectables títulos virales relativamente altos en el riñón, proventrículo, tonsilas cecales, íleo, recto y bolsa de Fabricio. La inmunofluorescencia demostró antígenos virales en los enterocitos de las extremidades de las vellosidades del íleo y recto y también en la bolsa. Se demostraron los antígenos virales en las células epiteliales de la tráquea y los túbulos renales.

Page Thumbnails

  • Thumbnail: Page 
809
    809
  • Thumbnail: Page 
810
    810
  • Thumbnail: Page 
811
    811
  • Thumbnail: Page 
812
    812
  • Thumbnail: Page 
813
    813
  • Thumbnail: Page 
814
    814
  • Thumbnail: Page 
815
    815
  • Thumbnail: Page 
816
    816
  • Thumbnail: Page 
817
    817