Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

If You Use a Screen Reader

This content is available through Read Online (Free) program, which relies on page scans. Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.

Phagocytosis and Intracellular Killing of Campylobacter jejuni by Elicited Chicken Peritoneal Macrophages

Margaret A. Myszewski and Norman J. Stern
Avian Diseases
Vol. 35, No. 4 (Oct. - Dec., 1991), pp. 750-755
DOI: 10.2307/1591606
Stable URL: http://www.jstor.org/stable/1591606
Page Count: 6
  • Read Online (Free)
  • Download ($12.00)
  • Subscribe ($19.50)
  • Cite this Item
Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Phagocytosis and Intracellular Killing of Campylobacter jejuni by Elicited Chicken Peritoneal Macrophages
Preview not available

Abstract

In vitro phagocytosis and intracellular survival of Campylobacter jejuni (strains B540 and Clin 1) in chicken peritoneal macrophages were studied. Macrophages were induced with Sephadex G-50 and harvested 48 hr later by peritoneal lavage. The extent of phagocytosis over time was determined by enumerating the intracellular C. jejuni after removal of extracellular C. jejuni with gentamicin. Pre-incubation of C. jejuni with antiserum generally enabled the macrophages to ingest greater numbers of cells than when the organism was pre-incubated in phosphate-buffered saline. C. jejuni were exposed to macrophage uptake for 30 minutes in a 5% CO2 incubator at 42 C. This suspension was then exposed to 12.5 μg gentamicin/ml to eliminate extracellular bacteria. Subsequently, the intracellular survival of C. jejuni was examined by monitoring its number within the macrophage at 30 minutes, 3 hr, and 6 hr after phagocytosis. Macrophages from C. jejuni-colonized chickens and from uncolonized control chickens were able to almost destroy the organism within the experimental period. /// Se estudió in vitro la fagocitosis y sobrevivencia de Campylobacter jejuni (cepas B540 y Clin 1) en macrófagos peritoneales de pollos. Los macrófagos fueron inducidos con Sefadex G-50 y obtenidos 48 horas después por lavado peritoneal. La duración de la fagocitosis fue determinada por recuento de C. jejuni intracelular después de remover el C. jejuni extracelular con gentamicina. La preincubación de C. jejuni con antisuero generalmente permitió a los macrófagos ingerir mayor cantidad de células que cuando los microorganismos fueron preincubados en una solución salina fosfatada. C. jejuni fue expuesto a la acción de los macrófagos durante 30 minutos a una temperatura de 42 C y una atmósfera de 5% de dióxido de carbono. Esta suspensión fue expuesta después a 12.5 μg/ml de gentamicina para eliminar las bacterias extracelulares. Seguidamente, la sobrevivencia intracelular del C. jejuni fue examinada mediante recuentos a los 30 minutos, 3 horas y 6 horas después de la fagocitosis. Los macrófagos de pollos colonizados con C. jejuni y aquellos de pollos controles no colonizados, fueron capaces de destruir los organismos durante el periodo experimental.

Page Thumbnails

  • Thumbnail: Page 
750
    750
  • Thumbnail: Page 
751
    751
  • Thumbnail: Page 
752
    752
  • Thumbnail: Page 
753
    753
  • Thumbnail: Page 
754
    754
  • Thumbnail: Page 
755
    755