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Effect of Mixed Cecal Microflora Maintained in Continuous Culture and of Dietary Lactose on Salmonella typhimurium Colonization in Broiler Chicks

David J. Nisbet, Donald E. Corrier and John R. DeLoach
Avian Diseases
Vol. 37, No. 2 (Apr. - Jun., 1993), pp. 528-535
DOI: 10.2307/1591682
Stable URL: http://www.jstor.org/stable/1591682
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Effect of Mixed Cecal Microflora Maintained in Continuous Culture and of Dietary Lactose on Salmonella typhimurium Colonization in Broiler Chicks
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Abstract

Mixed cecal microflora obtained from a mature chicken were maintained in vitro in continuous-flow (CF) culture. The effect of the CF culture and dietary lactose on Salmonella typhimurium cecal colonization in broiler chicks was evaluated. When averaged across four replicates, chicks treated with the culture alone (1.75 log10 decrease) or with 5% dietary lactose alone (2.98 log10 decrease) were protected against S. typhimurium. Optimum protection against S. typhimurium was observed when birds were treated with the culture in combination with dietary lactose (4.27 log10 decrease). Dietary lactose resulted in reduced cecal pH. A large increase in cecal propionic acid was observed in the birds given the CF culture. A significant correlation (P < 0.001) was observed between the cecal concentration of undissociated propionic acid and protection against S. typhimurium colonization (r = -0.78). The results indicated that indigenous cecal flora that protect against Salmonella colonization can be maintained without loss of efficacy in CF culture. /// Una mezcla de microflora cecal obtenida de un pollo adulto, fue mantenida in vitro en cultivo de flujo continuo. Se evaluó el efecto del cultivo y de la lactosa dietética sobre la colonización cecal por Salmonella typhimurium en pollos de engorde. Pollos tratados con el cultivo o con 5% de lactosa dietética, fueron protegidos contra S. typhimurium. La protección óptima contra S. typhimurium se observó cuando las aves fueron tratadas con el cultivo en combinación con la lactosa dietética. El tratamiento con la lactosa dietética resultó en la reducción del pH cecal. Se observó un gran aumento en el ácido propiónico cecal en las aves que recibieron el cultivo de flujo continuo. Se observó una correlación significante (P < 0.001) entre la concentración cecal de ácido propiónico no disociado y la protección frente a la colonización por S. typhimurium (r = -0.78). Los resultados indicaron que la flora cecal nativa que protege contra la colonización por Salmonella, puede mantenerse en cultivo de flujo continuo, sin pérdida de su eficacia.

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