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The Effect of Exposure, Storage Times, and Types of Holding Media on the Drag-Swab Monitoring Technique for Salmonella

O. O. Opara, E. T. Mallinson, C. R. Tate, L. E. Carr, R. G. Miller, L. Stewart, C. Kelleher, R. W. Johnston and S. W. Joseph
Avian Diseases
Vol. 36, No. 1 (Jan. - Mar., 1992), pp. 63-68
DOI: 10.2307/1591717
Stable URL: http://www.jstor.org/stable/1591717
Page Count: 6
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
The Effect of Exposure, Storage Times, and Types of Holding Media on the Drag-Swab Monitoring Technique for Salmonella
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Abstract

Four maintenance media were compared for the preservation of the sensitivity of drag swabs and to assess the survival of Salmonella spp. on drag swabs at reduced temperatures. The effects of Difco double-strength skim milk (2 × SM), 2% buffered peptone water (BPW), a modified liquid Cary-Blair transport medium (LCB), and lactose broth holding medium were compared, as were storage periods of various lengths. The results with enzyme-linked immunosorbent antigen capture assay and highly selective plating media detection systems showed that 2 × SM had the highest level of recovery of salmonellae after prolonged storage, both under refrigeration at 4 C for 3 days and under frozen conditions of - 15 C for 2 weeks. /// Se compararon cuatro medios de mantenimiento diferentes para la preservación de la sensibilidad de la técnica del hisopo y se evaluó la sobrevivencia de Salmonella spp. en los hisopos mantenidos a bajas temperaturas. Se compararon los efectos de los siguientes medios de mantenimiento: leche descremada de Difco usada en doble concentración, agua peptonada buferada al 2%, líquido modificado de Cary-Blair (medio de transporte), y caldo lactosado. Se compararon también varios períodos de almacenamiento. Los resultados con la prueba ELISA para captura del antígeno y los sistemas de detección en medio altamente selectivo, mostraron que la leche descremada tenía una capacidad más alta de aislar Salmonella después de un prolongado almacenamiento en refrigeración a 4 C durante 3 días y en congelamiento a - 15 C por dos semanas.

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