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Antigenic Relatedness between Seven Avian Mycoplasma Species as Revealed by Western Blot Analysis

B. Ben Abdelmoumen and R. S. Roy
Avian Diseases
Vol. 39, No. 2 (Apr. - Jun., 1995), pp. 250-262
DOI: 10.2307/1591866
Stable URL: http://www.jstor.org/stable/1591866
Page Count: 13
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Antigenic Relatedness between Seven Avian Mycoplasma Species as Revealed by Western Blot Analysis
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Abstract

Cross-reactivities among seven avian mycoplasma species (Mycoplasma gallisepticum, M. synoviae, M. meleagridis, M. iowae, M. anatis, M. gallinarum, and M. iners) were examined using rabbit polyclonal antisera and two monoclonal antibodies (MAbs) against M. pneumoniae: Myc-9 and Myc-4. The major antigens and cross-reacting antigens of the seven mycoplasma species were demonstrated by immunoblotting assay using homologous and heterologous antisera. Two-way cross-reacting antigens of MS and MG, with apparent molecular weights of 44,000 and 36,000, were identified. Additionally, the 36,000 protein of MG was recognized by all of the heterologous antisera. A large number of cross-reactivities, which have been never investigated before, were shown between M. meleagridis and M. synoviae, between M. meleagridis and M. gallinarum, between M. meleagridis and M. iners, and between M. gallinarum and M. iners. All of the cross-reactions existing between the majority of the above species were considerably reduced or eliminated by adsorbing the polyclonal antisera with a pool of heterologous antigens. Two MAbs against a M. pneumoniae protein of a molecular weight of 150,000 were also able to recognize several avian mycoplasma epitopes, of which two common epitopes with molecular weights of 44,000 and 42,000 were present in most of the seven mycoplasma species. /// Se examinaron las reacciones cruzadas de siete especies de micoplasmas aviares (Mycoplasma gallisepticum, M. synoviae, M. meleagridis, M. iowae, M. anatis, M. gallinarum y M. iners) utilizando antisuero policlonal de conejo y dos anticuerpos monoclonales contra M. pneumoniae: Myc-9 y Myc-4. Se demostró la presencia de los antígenos principales y de los antígenos de reacción cruzada usando la inmunotransferencia puntual con antisueros homólogos y heterólogos. Se identificaron antígenos de reacción cruzada entre MS y MG, con pesos moleculares de 44,000 y 36,000, respectivamente. Adicionalmente, la proteína de MG de peso molecular 36,000 fue reconocida por todos los antisueros heterólogos. Se demostró la presencia de un gran número de reacciones cruzadas que no habían sido investigadas anteriormente, entre: M. meleagridis y M. synoviae, M. meleagridis y M. gallinarum, M. meleagridis y M. iners, y entre M. gallinarum y M. iners. Todas las reacciones cruzadas existentes entre la mayoría de las especies indicadas se redujeron considerablemente o fueron eliminadas mediante la adsorción del antisuero policlonal con una mezcla de los antígenos heterólogos. Dos anticuerpos monoclonales contra una proteína del M. pneumoniae de peso molecular 150,000 también reconocieron varios epítopes de varias especies de micoplasmas aviares, entre los cuales los dos epítopes más comunes presentes en la mayoría de las siete especies de micoplasma tuvieron pesos moleculares de 44,000 y 42,000.

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