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Evaluation of Possible Alternatives to Double-Strength Skim Milk Used to Saturate Drag Swabs for Salmonella Detection

O. O. Opara, L. E. Carr, C. R. Tate, R. G. Miller, E. T. Mallinson, L. E. Stewart and S. W. Joseph
Avian Diseases
Vol. 38, No. 2 (Apr. - Jun., 1994), pp. 293-296
DOI: 10.2307/1591952
Stable URL: http://www.jstor.org/stable/1591952
Page Count: 4
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Evaluation of Possible Alternatives to Double-Strength Skim Milk Used to Saturate Drag Swabs for Salmonella Detection
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Abstract

The drag-swab Salmonella screening technique was evaluated using less expensive alternatives to double-strength skim milk (2×SM) as a saturating medium for drag swabs. Ten pre-determined Salmonella-positive poultry houses were studied. In the first phase, Salmonella screening efficiency of drag swabs impregnated with 2×SM and commercially available canned Carnation® evaporated skim milk (CESM) were compared. Results showed CESM to be a less efficient alternative. In the second phase of the study, the Salmonella screening efficiency of drag swabs impregnated with 2% buffered peptone water (BPW), physiological saline (PS), and distilled water (DW) were evaluated along with an unimpregnated drag swab (dry drag swab) (DD) as possible alternatives to 2×SM. The efficiency of Salmonella detection using various impregnation treatments were in the following order: 2×SM > PS > BPW > DW > DD. /// Se evaluó la técnica para la detección de Salmonella utilizando hisopos de arrastre, usando una alternativa menos costosa a la leche descremada a concentración doble como un medio para saturar los hisopos de arrastre. Se estudiaron 10 galpones con aves positivas a Salmonella. En la primera fase se comparó la eficiencia de los hisopos de arrastre impregnados con leche descremada frente a la leche evaporada enlatada (Carnation®). Los resultados mostraron que la leche evaporada enlatada es una alternativa menos eficiente. En la segunda fase del estudio, la eficiencia de los hisopos impregnados con agua peptonada buferada al 2%, solución salina fisiológica y agua destilada, fueron evaluados junto con un hisopo de arrastre seco, comparando con la leche descremada como posibles alternativas. La eficiencia de la detección de Salmonella usando los diferentes tratamientos fue en el siguiente orden: Leche descremada a concentración doble, solución salina buferada, agua peptonada buferada al 2%, agua destilada e hisopo seco.

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