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Antigenic Characterization of a Turkey Coronavirus Identified in Poult Enteritis- and Mortality Syndrome-Affected Turkeys

James S. Guy, H. John Barnes, Lynda G. Smith and Jamie Breslin
Avian Diseases
Vol. 41, No. 3 (Jul. - Sep., 1997), pp. 583-590
DOI: 10.2307/1592148
Stable URL: http://www.jstor.org/stable/1592148
Page Count: 8
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Antigenic Characterization of a Turkey Coronavirus Identified in Poult Enteritis- and Mortality Syndrome-Affected Turkeys
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Abstract

A turkey coronavirus (TCV [NC95]) was characterized by antigenic comparison with other avian and mammalian coronaviruses using immunofluorescence (FA) and immunoperoxidase (IP) procedures. Based on FA and IP procedures, TCV (NC95) was determined to be antigenically indistinguishable from turkey enteric (bluecomb) coronavirus (TECV). In addition, TCV (NC95) and TECV were found to be closely related to infectious bronchitis virus (IBV); a one-way antigenic relationship was demonstrated. Polyclonal antibodies specific for TECV and IBV reacted strongly against TCV (NC95), as determined by FA procedures. Monoclonal antibodies (MAbs) specific for IBV matrix protein (MAb 919) reacted strongly against TCV (NC95) and TECV as determined by FA and IP procedures; an IBV peplomer protein-specific MAb (MAb 94) did not recognize the two viruses. These studies suggest an identification of TCV (NC95) as a strain of TECV, and provide evidence of a close antigenic relationship between these viruses and IBV. /// Se caracterizó un coronavirus de pavos identificado como NC95 mediante la comparación antigénica con otros coronavirus aviares y de mamíferos, utilizando las pruebas de inmunofluorescencia e inmunoperoxidasa. Basados en estas dos pruebas, se determinó que el coronavirus de pavos NC95 es antigénicamente indiferenciable del coronavirus entérico de pavos que produce la cresta azul. Además, se encontró que el coronavirus de pavo NC95 y el coronavirus entérico de pavos tienen una relación antigénicamente estrecha en una dirección con el virus de bronquitis infecciosa. Por medio de la inmunofluorescencia se determinó que anticuerpos policlonales específicos contra el coronavirus entérico de pavos y el virus de bronquitis infecciosa reaccionan fuertemente contra el coronavirus de pavos NC95. Por medio de las pruebas de inmunofluorescencia e inmunoperoxidasa se determinó que el anticuerpo monoclonal 919 específico contra la proteína matriz del virus de bronquitis infecciosa reaccionó fuertemente contra el coronavirus de pavos NC95 y contra el coronavirus entérico de pavos. El anticuerpo monoclonal 94 específico contra un peplómero del virus de bronquitis infecciosa no reconoció los dos virus. Estos resultados sugieren que el coronavirus de pavos NC95 es una cepa del coronavirus entérico de pavos y suministran evidencia de que existe una relación antigénica muy cercana entre estos dos virus y el virus de bronquitis.

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