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Comparison of Several Enzymes between Normal Physeal and Tibial Dyschondroplastic Cartilage of Broiler Chickens

X. Zhang, H. Huang, G. R. McDaniel and J. J. Giambrone
Avian Diseases
Vol. 41, No. 2 (Apr. - Jun., 1997), pp. 330-334
DOI: 10.2307/1592186
Stable URL: http://www.jstor.org/stable/1592186
Page Count: 5
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Comparison of Several Enzymes between Normal Physeal and Tibial Dyschondroplastic Cartilage of Broiler Chickens
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Abstract

Normal physeal and dyschondroplastic cartilage of broiler chickens was examined for six enzymes by isoelectric focusing in thin-layer polyacrylamide slab gels. Acid phosphatase (ACP), esterase (EST), malate dehydrogenase (MDH), and peroxidase (PRX) were present in the normal physeal cartilage but not in the dyschondroplastic cartilage. Staining intensity of glucose-6-phosphate isomerase (GPI) and triose-phosphate isomerase (TPI) was reduced in the dyschondroplastic cartilage compared with that of the physeal cartilage. Differences in the presence of these enzymes possibly demonstrated their roles in processes of bone formation, cartilage resorption, and calcification. ACP could be involved in calcification. Lack of EST and PRX may be related to the failure of vascular invasion in dyschondroplastic cartilage of afflicted birds. A deficiency of MDH and reduced GPI and TPI in dyschondroplastic cartilage may reflect a reduction in the activity of energetic metabolism, causing the dissipation of energy and necrotic cells. /// Usando seis enzimas, se examinaron las placas epifisiarias cartilaginosas de crecimiento normales y discondroplásticas de la tibia de pollos de engorde, mediante la técnica del foco isoeléctrico sobre capas delgadas de geles de poliacrilamida. La fosfatasa ácida, la esterasa, la dehidrogenasa malato y la peroxidasa estaban presentes en la placa de crecimiento cartilaginoso normal pero no en el cartílago discondroplástico. La intensidad de la coloración de las isomerasas glucosa-6 fosfato y triosa-fosfato fue menor en el cartílago discondroplástico que en la placa de crecimiento cartilaginoso normal. Las diferencias en la presencia de estas enzimas posiblemente demonstraron su papel en el proceso de la formación del hueso, readsorción del cartílago y calcificación. La fosfatasa ácida podría estar involucrada en el proceso de calcificación. La ausencia de esterasa y peroxidasa pueden estar relacionadas con las fallas de vascularización del cartílago discondroplástico de las aves afectadas. La deficiencia de la dehidrogenasa maleato y la disminución de las isomerasas glucosa-6-fosfato y triosa-fosfato en el cartílago discondroplástico pueden reflejar una reducción en la actividad del metabolismo energético, causando el agotamiento de energía y la necrosis de las células.

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