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Journal Article

Application of Polymerase Chain Reaction with Arbitrary Primers to Strain Identification of Mycoplasma gallisepticum

H. H. Fan, S. H. Kleven and M. W. Jackwood
Avian Diseases
Vol. 39, No. 4 (Oct. - Dec., 1995), pp. 729-735
DOI: 10.2307/1592409
Stable URL: http://www.jstor.org/stable/1592409
Page Count: 7
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Since scans are not currently available to screen readers, please contact JSTOR User Support for access. We'll provide a PDF copy for your screen reader.
Application of Polymerase Chain Reaction with Arbitrary Primers to Strain Identification of Mycoplasma gallisepticum
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Abstract

DNA heterogeneity among strains and isolates of Mycoplasma gallisepticum (MG) was demonstrated with the arbitrarily primed polymerase chain reaction (AP-PCR) method. This method involves three cycles of low-stringency amplification followed by PCR at higher stringency. Reproducible DNA fragments of 25 different MG strains or isolates were generated with three arbitrarily chosen primers. The MG strains or isolates were distinguished according to the banding patterns of their amplified DNA on agarose gels, and the differences were characteristic for specific isolates. This method is rapid, simple, and reproducible, and it can also be used to determine the similarity between isolates of MG from various sources. /// La heterogenicidad y la relación entre las cepas de Mycoplasma gallisepticum (MG) fue demostrada con la reacción en cadena por la polimerasa y con iniciadores escogidos al azar. Este método comprende tres ciclos de amplificación de baja restricción, seguido por una reacción en cadena por la polimerasa de alta restricción. Se obtuvieron fragmentos de DNA reproducibles de 25 cepas diferentes de MG con tres iniciadores escogidos al azar. Las cepas de MG fueron diferenciadas en geles de agarosa de acuerdo con los patrones de las bandas del DNA amplificado, y las diferencias fueron características para cada cepa específica. Este método es rápido, simple y reproducible y puede ser usado también para determinar la similitud entre cepas de MG de varias fuentes.

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