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Experimental Bovine Coronavirus in Turkey Poults and Young Chickens

M. M. Ismail, K. O. Cho, L. A. Ward, L. J. Saif and Y. M. Saif
Avian Diseases
Vol. 45, No. 1 (Jan. - Mar., 2001), pp. 157-163
DOI: 10.2307/1593023
Stable URL: http://www.jstor.org/stable/1593023
Page Count: 7
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Experimental Bovine Coronavirus in Turkey Poults and Young Chickens
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Abstract

The DB2 calf strain of bovine coronavirus (BCV) was used to inoculate 1-day-old specific-pathogen-free (SPF) turkey poults in three trials. In all trials, the birds developed clinical signs of enteritis at 48-72 hr postinoculation. Birds euthanatized at 3, 5, and 7 days postinoculation (DPI) had flaccid, pale intestines with watery contents, and the ceca were markedly enlarged with frothy contents. Coronavirus particles were detected by immune electron microscopy with BCV antibodies from the intestinal contents of birds killed at 3, 5, 7, and 12 DPI. Body weights of inoculated poults killed at 3, 5, and 7 DPI were significantly reduced as compared with controls. Hemagglutinating antibodies were detected in sera of convalescent birds at 12 DPI. However, experimental inoculation of 1-day-old SPF chicks in two trials with the same virus resulted in no clinical signs or macroscopic or microscopic lesions. No coronaviruses were detected from intestinal contents, and there were no significant differences in body weights of inoculated and noninoculated control chicks. /// Se utilizó la cepa DB2 del coronavirus bovino para inocular experimentalmente pavitos libres de patógenos específicos de un día de edad. En tres experimentos realizados, las aves desarrollaron signos clínicos de enteritis de las 48 a las 72 horas después de la inoculación. Las aves sacrificadas a los 3, 5 y 7 días después de la inoculación tenían intestinos pálidos, fláccidos con contenido acuoso y el ciego estaba severamente agrandado con contenido espumoso. Por medio de la inmunomicroscopía electrónica utilizando anticuerpos de coronavirus bovino, se detectaron partículas de coronavirus en el contenido intestinal de las aves sacrificadas a los 3, 5, 7 y 12 días después de la inoculación. El peso corporal de los pavitos inoculados sacrificados a los 3, 5 y 7 días después de la inoculación estuvo bastante reducido en comparación con el de los controles. En el suero de aves convalescientes se detectaron anticuerpos hemaglutinantes a los 12 días después de la inoculación. Sin embargo, en dos experimentos la inoculación de pollitos libres de patógenos específicos de un día de edad con el mismo virus no produjo signos clínicos ni lesiones macroscópicas ó microscópicas. No se observó el coronavirus en el contenido intestinal y no hubo diferencias significantes en el peso corporal de los pollitos inoculados y los controles no inoculados.

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