Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Studies on Insulin Biosynthesis. Subcellular Distribution of Leucine-H 3 Radioactivity during Incubation of Goosefish Islet Tissue

G. Eric Bauer, Arnold W. Lindall, Jr., Padmaker K. Dixit, Gordon Lester and Arnold Lazarow
The Journal of Cell Biology
Vol. 28, No. 3 (Mar., 1966), pp. 413-421
Stable URL: http://www.jstor.org/stable/1604819
Page Count: 9
  • More info
  • Cite this Item
Studies on Insulin Biosynthesis. Subcellular Distribution of Leucine-H
          3
          Radioactivity during Incubation of Goosefish Islet Tissue
Preview not available

Abstract

Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H3. The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules.

Page Thumbnails

  • Thumbnail: Page 
413
    413
  • Thumbnail: Page 
414
    414
  • Thumbnail: Page 
415
    415
  • Thumbnail: Page 
416
    416
  • Thumbnail: Page 
417
    417
  • Thumbnail: Page 
418
    418
  • Thumbnail: Page 
419
    419
  • Thumbnail: Page 
420
    420
  • Thumbnail: Page 
421
    421