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Purification of Intact Microtubules from Brain

Joel B. Kirkpatrick, Lyon Hyams, Virginia L. Thomas and Peter M. Howley
The Journal of Cell Biology
Vol. 47, No. 2, Part 1 (Nov., 1970), pp. 384-394
Stable URL: http://www.jstor.org/stable/1606093
Page Count: 11
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Purification of Intact Microtubules from Brain
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Abstract

Hundred-fold purification of intact microtubules from homogenates of rat brain is reported. The success of purification depends on stabilizing the microtubule structure by the combined effects of hexylene glycol, acidic pH, and low temperature. A practical, negative stain, electron microscopic assay is used to study purity and stability of microtubule fractions. The purified fractions show a major band which migrates like purified tubulin in the SDS gel electrophoresis system.

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