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Fractionation of the Nucleus by Divalent Cations: Isolation of Nuclear Membranes

Ariane Monneron, Günter Blobel and George E. Palade
The Journal of Cell Biology
Vol. 55, No. 1 (Oct., 1972), pp. 104-125
Stable URL: http://www.jstor.org/stable/1606776
Page Count: 22
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Fractionation of the Nucleus by Divalent Cations: Isolation of Nuclear Membranes
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Abstract

0.3-0.5 M MgCl2 was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55-60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.

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