Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Cell Mitotic Cycle Synthesis of Nil Hamster Glycolipids Including the Forssman Antigen

Barbara Anne Wolf and P. W. Robbins
The Journal of Cell Biology
Vol. 61, No. 3 (Jun., 1974), pp. 676-687
Stable URL: http://www.jstor.org/stable/1607103
Page Count: 12
  • More info
  • Cite this Item
Cell Mitotic Cycle Synthesis of Nil Hamster Glycolipids Including the Forssman Antigen
Preview not available

Abstract

The synthesis of phospholipids and glycolipids during the cell mitotic cycle of an established hamster line, NIL, has been studied. Cells were synchronized with excess thymidine and mitotically harvested by shaking. Cells were radioactively labeled for 4 h with palmitate, glucosamine, or galactose. Lipids were analyzed by thin-layer chromatography. As cells progressed through the mitotic cycle, incorporation into phospholipids increased but the fraction represented by each remained constant. Similarly, ceramide monohexoside, dihexoside, and hematoside were labeled equally in all phases. Ceramide trihexoside and tetrahexoside were labeled only during G1 and S. Ceramide pentahexoside (the Forssman antigen) shows density-dependent synthesis, accumulation, and reactivity. Ceramide pentahexoside was labeled during all phases of the mitotic cycle but the rate of incorporation decreased in S and G2. The total amount of lipid assayed immunologically in cell extracts gradually increased. Exposure of the Forssman antigen in untreated or trypsin-treated cells was studied using binding of chemically labeled antiForssman antiserum. The amount of antigen detected in trypsinized cells increased during G1 and early S but then remained constant. Mitotic cells exposed all detectable antigen. As cells progressed through the mitotic cycle, a large fraction of the Forssman antigen became cryptic.

Page Thumbnails

  • Thumbnail: Page 
676
    676
  • Thumbnail: Page 
677
    677
  • Thumbnail: Page 
678
    678
  • Thumbnail: Page 
679
    679
  • Thumbnail: Page 
680
    680
  • Thumbnail: Page 
681
    681
  • Thumbnail: Page 
682
    682
  • Thumbnail: Page 
683
    683
  • Thumbnail: Page 
684
    684
  • Thumbnail: Page 
685
    685
  • Thumbnail: Page 
686
    686
  • Thumbnail: Page 
687
    687