Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

The Effects of Halothane on Cultured Mouse Neuroblastoma Cells. I. Inhibition of Morphological Differentiation

Robert E. Hinkley and Alvin G. Telser
The Journal of Cell Biology
Vol. 63, No. 2, Part 1 (Nov., 1974), pp. 531-540
Stable URL: http://www.jstor.org/stable/1607387
Page Count: 10
  • More info
  • Cite this Item
The Effects of Halothane on Cultured Mouse Neuroblastoma Cells. I. Inhibition of Morphological Differentiation
Preview not available

Abstract

Mouse neuroblastoma cells (clone NB2a) were cultured in the presence of 0.3-2.1% halothane in the gas phase for up to 72 h. Halothane inhibited neurite extension dose dependently and virtually abolished microspike formation even at the lowest concentration tested. These effects were completely reversible. Electron microscopy demonstrated that microfilaments measuring 40-80 Å in diameter are the only fibrous organelles visible within microspikes. When the cells were exposed to halothane, no microfilamentous complexes could be identified in any cells and the subcortical regions of neurites often appeared devoid of individual microfilaments. Microtubules were still present in neurites after exposure to halothane concentrations at which microfilaments disappeared. However, at concentrations above 1.0%, microtubules gradually appeared to decrease in number. Short-term experiments showed that existing neurites and microspikes rapidly retracted when suddenly exposed to culture medium equilibrated with 1.0% halothane and quickly reformed when the halothane was removed. The inhibition of neuroblastoma cell differentiation by halothane appears to be mediated by disruption of 40-80 Å diameter microfilaments.

Page Thumbnails

  • Thumbnail: Page 
531
    531
  • Thumbnail: Page 
532
    532
  • Thumbnail: Page 
[533]
    [533]
  • Thumbnail: Page 
534
    534
  • Thumbnail: Page 
535
    535
  • Thumbnail: Page 
536
    536
  • Thumbnail: Page 
537
    537
  • Thumbnail: Page 
538
    538
  • Thumbnail: Page 
539
    539
  • Thumbnail: Page 
540
    540