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Troponin in Embryonic Chick Skeletal Muscle Cells in vitro: An Immunoelectron Microscope Study

Takashi Obinata, Yutaka Shimada and Ryoichi Matsuda
The Journal of Cell Biology
Vol. 81, No. 1 (Apr., 1979), pp. 59-66
Stable URL: http://www.jstor.org/stable/1608736
Page Count: 8
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Troponin in Embryonic Chick Skeletal Muscle Cells in vitro: An Immunoelectron Microscope Study
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Abstract

The fine structural distribution of troponin on thin filaments in developing myofibrils was investigated by the use of immunoelectron microscopy. Embryonic chick skeletal muscle cells grown in vitro were treated with antibodies against each of the troponin components (troponin T, I, and C) from adult chicken muscles. Each antibody was distributed along the thin filaments with a period of 38 nm. It is concluded that these newly synthesized regulatory proteins are assembled at their characteristic position from the initial phases of myofibrillogenesis.

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