Access

You are not currently logged in.

Access your personal account or get JSTOR access through your library or other institution:

login

Log in to your personal account or through your institution.

Studies of Schwann Cell Proliferation: I. An Analysis in Tissue Culture of Proliferation during Development, Wallerian Degeneration, and Direct Injury

James L. Salzer and Richard P. Bunge
The Journal of Cell Biology
Vol. 84, No. 3 (Mar., 1980), pp. 739-752
Stable URL: http://www.jstor.org/stable/1608847
Page Count: 14
  • More info
  • Cite this Item
Studies of Schwann Cell Proliferation: I. An Analysis in Tissue Culture of Proliferation during Development, Wallerian Degeneration, and Direct Injury
Preview not available

Abstract

In this paper the stimuli for and pattern of Schwann cell proliferation are defined under various experimental conditions. We used a tissue culture system in which fetal rat dorsal root ganglia, treated to eliminate contaminating fibroblasts (Wood, P., 1976, Brain Res. 115:361-375), appear to recapitulate many aspects of the developing peripheral nervous system. We observed that: (a) proliferation of Schwann cells on neurites is initially rapid, but, as each neurite becomes fully ensheathed, division slows considerably and is confined to the periphery of the outgrowth; (b) during the period of rapid proliferation, excision of the ganglion causes a rapid decay in the number of dividing cells; (c) excision of the ganglion from more established cultures in which there was little ongoing proliferation resulted in a small increase in labeling at the site of excision for all Schwann cells and a substantial increase in labeling for myelin-related cells with a peak labeling period at 4 d; (d) direct mechanical injury during Wallerian degeneration is mitogenic for Schwann cells; (e) a variety of potential mitogens failed to stimulate Schwann cell proliferation; and (f) replated cells have a slightly higher level of proliferation and show a small and variable response to the addition of cAMP.

Page Thumbnails

  • Thumbnail: Page 
739
    739
  • Thumbnail: Page 
740
    740
  • Thumbnail: Page 
741
    741
  • Thumbnail: Page 
742
    742
  • Thumbnail: Page 
743
    743
  • Thumbnail: Page 
744
    744
  • Thumbnail: Page 
[745]
    [745]
  • Thumbnail: Page 
746
    746
  • Thumbnail: Page 
747
    747
  • Thumbnail: Page 
748
    748
  • Thumbnail: Page 
749
    749
  • Thumbnail: Page 
750
    750
  • Thumbnail: Page 
751
    751
  • Thumbnail: Page 
752
    752